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鈴木 宏和（すずき ひろかず）
1975年10月17日 生まれ，東京都出身，博士（工学）
E-mail: hirokazusuzuki[at]tottori-u.ac.jp

ゲーム機の進化に伴いスゴいゲームはつくれるようになったが，面白いゲームがつくれるとは限らない。あるゲームクリエーターの言葉です。かなり昔に聞いたものですが，今も不思議と覚えています。応用微生物学分野や酵素工学分野において，同様な発言ができることを夢見て精進しています。また一生涯，ウェットな現場に立ち続けることも目指しています。

経歴
1999年 3月  　東北大学 工学部 生物化学工学科 卒業
2001年 3月  　東北大学大学院 工学研究科 生物工学専攻 博士前期課程 修了
2004年 3月  　東北大学大学院 工学研究科 生物工学専攻 博士後期課程 修了，博士（工学）
2004年 4月  　東京大学大学院 農学生命科学研究科 醗酵学研究室 博士研究員
2007年 4月  　理化学研究所 長田抗生物質研究室 基礎科学特別研究員
2008年 10月  　神戸大学 自然科学系先端融合研究環 重点研究部 特命助教
2011年 4月  　九州大学大学院 農学研究院 寄附講座 客員准教授
2014年 4月  　鳥取大学大学院 工学研究科 准教授（現職）
所属学会
  1. 日本農芸化学会
  2. 日本生物工学会
  3. 極限環境生物学会
  4. アメリカ微生物学会
原著論文
  1. Saori Kani, Hirokazu Suzuki. Whole genome sequence of Geobacillus thermodenitrificans K1041, a genetically tractable strain representative of the genus Geobacillus. Microbiol. Resour. Announc. 13, e0084823 (2024)
  2. Ryota Kurashiki, Kosuke Koyama, Yukina Sakaguchi, Yuta Okumura, Takashi Ohshiro, Hirokazu Suzuki. Development of a thermophilic host-vector system for the production of recombinant proteins at elevated temperatures. Appl. Microbiol. Biotechnol. 107, 7475-7488 (2023)
  3. Kosuke Koyama, Yui Mikawa, Shota Nakagawa, Ryota Kurashiki, Takashi Ohshiro, Hirokazu Suzuki. New platform for screening genetic libraries at elevated temperatures: biological and genomic information and genetic tools of Geobacillus thermodenitrificans K1041. Appl. Environ. Microbiol. 88, e0105122 (2022)
  4. Yoshihito Arai, Yunako Shingu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Occurrence of different fucoidanase genes in Flavobacterium sp. SW and enzyme characterization. J. Biosci. Bioeng. 134, 187-194 (2022)
  5. Hirokazu Suzuki, Yuta Okumura, Yui Mikawa, Mao Takata, Shunsuke Yoshimura, Takashi Ohshiro. Transcriptome and growth efficiency comparisons of recombinant thermophiles that produce thermolabile and thermostable proteins: implications for burden-based selection of thermostable proteins. Extremophiles 25, 403-412 (2021)
  6. Hirokazu Suzuki, Tatsunari Taketani, Misaki Tanabiki, Misaki Ohara, Jyumpei Kobayashi, Takashi Ohshiro. Frequent transposition of multiple insertion sequences in Geobacillus kaustophilus HTA426. Front. Microbiol. 12, 650461 (2021)
  7. Ryota Kurashiki, Tatsuki Mizuno, Kurumi Murata, Takashi Ohshiro, Hirokazu Suzuki. A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species. Extremophiles 24, 147-156 (2020)
  8. Hirokazu Suzuki, Tomoaki Abe, Katsumi Doi, Toshihisa Ohshima. Azoreductase from alkaliphilic Bacillus sp. AO1 catalyzes indigo reduction. Appl. Microbiol. Biotechnol. 102, 9171-9181 (2018)
  9. Tatsuhiko Nagao, Yoshihito Arai, Mika Yamaoka, Fumika Komatsu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Identification and characterization of the fucoidanase gene from Luteolibacter algae H18. J. Biosci. Bioeng. 126, 567-572 (2018)
  10. Kenta Fujii, Yurie Tominaga, Jyumpei Okunaka, Hisashi Yagi, Takashi Ohshiro, Hirokazu Suzuki. Microbial and genomic characterization of Geobacillus thermodenitrificans OS27, a marine thermophile that degrades diverse raw seaweeds. Appl. Microbiol. Biotechnol. 102, 4901-4913 (2018)
  11. Hirokazu Suzuki, Tatsunari Taketani, Jyumpei Kobayashi, Takashi Ohshiro. Antibiotic resistance mutations induced in growing cells of Bacillus-related thermophiles. J. Antibiot. 71, 382-389 (2018)
  12. Tomoya Hino, Haruka Hamamoto, Hirokazu Suzuki, Hisashi Yagi, Takashi Ohshiro, Shingo Nagano. Crystal structures of TdsC, a dibenzothiophene monooxygenase from the thermophile Paenibacillus sp. A11-2, reveal potential for expanding its substrate selectivity. J. Biol. Chem. 292, 15804-15813 (2017)
  13. Tatsuhiko Nagao, Ayako Kumabe, Fumika Komatsu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Gene identification and characterization of fucoidan deacetylase for potential application to fucoidan degradation and diversification. J. Biosci. Bioeng. 124, 277-282 (2017)
  14. Yurie Tominaga, Takashi Ohshiro, Hirokazu Suzuki. Conjugative plasmid transfer from Escherichia coli is a versatile approach for genetic transformation of thermophilic Bacillus and Geobacillus species. Extremophiles 20, 375-381 (2016)
  15. Keisuke Wada, Jyumpei Kobayashi, Megumi Furukawa, Katsumi Doi, Takashi Ohshiro, Hirokazu Suzuki. A thiostrepton resistance gene and its mutants serve as selectable markers in Geobacillus kaustophilus HTA426. Biosci. Biotechnol. Biochem. 80, 368-375 (2016)
  16. Jyumpei Kobayashi, Misaki Tanabiki, Shohei Doi, Akihiko Kondo, Takashi Ohshiro, Hirokazu Suzuki. Unique plasmids generated via pUC replicon mutagenesis in an error-prone thermophile derived from Geobacillus kaustophilus HTA426. Appl. Environ. Microbiol. 81, 7625-7632 (2015)
  17. Jyumpei Kobayashi, Megumi Furukawa, Takashi Ohshiro, Hirokazu Suzuki. Thermoadaptation-directed evolution of chloramphenicol acetyltransferase in an error-prone thermophile using improved procedures. Appl. Microbiol. Biotechnol. 99, 5563-5572 (2015)
  18. Jyumpei Kobayashi, Jotaro Yukimoto, Yasuhiro Shimizu, Taketo Ohmori, Hirokazu Suzuki, Katsumi Doi, Toshihisa Ohshima. Characterization of Lactobacillus salivarius alanine racemase: short-chain carboxylate-activation and the role of A131. SpringerPlus 4, 639 (2015)
  19. Hirokazu Suzuki, Jyumpei Kobayashi, Keisuke Wada, Megumi Furukawa, Katsumi Doi. Thermoadaptation-directed enzyme evolution in an error-prone thermophile derived from Geobacillus kaustophilus HTA426. Appl. Environ. Microbiol. 81, 149-158 (2015)
  20. Hirokazu Suzuki, Jun Ishii, Akihiko Kondo, Ken-ichi Yoshida. Polyamino acid display on cell surfaces enhances salt and alcohol tolerance of Escherichia coli. Biotechnol. Lett. 37, 429-435 (2015)
  21. Hironaga Akita, Yoshifumi Imaizumi, Hirokazu Suzuki, Katsumi Doi, Toshihisa Ohshima. Spectrophotometric assay of D-isoleucine using an artificially created D-amino acid dehydrogenase. Biotechnol. Lett. 36, 2245-2248 (2014)
  22. Hironaga Akita, Hirokazu Suzuki, Katsumi Doi, Toshihisa Ohshima. Efficient synthesis of D-branched-chain amino acids and their labeled compounds with stable isotopes using D-amino acid dehydrogenase. Appl. Microbiol. Biotechnol. 98, 1135-1143 (2014)
  23. Hirokazu Suzuki, Keisuke Wada, Megumi Furukawa, Katsumi Doi, Toshihisa Ohshima. A ternary conjugation system for the construction of DNA libraries for Geobacillus kaustophilus HTA426. Biosci. Biotechnol. Biochem. 77, 2316-2318 (2013)
  24. Hirokazu Suzuki, Ayano Murakami, Ken-ichi Yoshida. Motif-guided identification of a glycoside hydrolase family 1 α-L-arabinofuranosidase in Bifidobacterium adolescentis. Biosci. Biotechnol. Biochem. 77, 1709-1714 (2013)
  25. Hirokazu Suzuki, Ken-ichi Yoshida, Toshihisa Ohshima. Polysaccharide-degrading thermophiles generated by heterologous gene expression in Geobacillus kaustophilus HTA426. Appl. Environ. Microbiol. 79, 5151-5158 (2013); this paper has been selected as an AEM Spotlight.
  26. Naoki Kato, Hirokazu Suzuki, Hideo Okumura, Shunji Takahashi, Hiroyuki Osada. A point mutation in ftmD blocks the fumitremorgin biosynthetic pathway in Aspergillus fumigatus strain Af293. Biosci. Biotechnol. Biochem. 77, 1061-1067 (2013)
  27. Hirokazu Suzuki, Fumiyoshi Okazaki, Akihiko Kondo, Ken-ichi Yoshida. Genome mining and motif modifications of glycoside hydrolase family 1 members encoded by Geobacillus kaustophilus HTA426 provide thermostable 6-phospho-β-glycosidase and β-fucosidase. Appl. Microbiol. Biotechnol. 97, 2929-2938 (2013)
  28. Hirokazu Suzuki, Ayano Murakami, Ken-ichi Yoshida. Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR. Appl. Environ. Microbiol. 78, 7376-7383 (2012)
  29. Hirokazu Suzuki, Ken-ichi Yoshida. Genetic transformation of Geobacillus kaustophilus HTA426 by conjugative transfer of host-mimicking plasmids. J. Microbiol. Biotechnol. 22, 1279-1287 (2012)
  30. Ken-ichi Yoshida, Azusa Sanbongi, Ayano Murakami, Hirokazu Suzuki, Shinji Takenaka, Hideto Takami. Three inositol dehydrogenases involved in utilization and interconversion of inositol stereoisomers in a thermophile Geobacillus kaustophilus HTA426. Microbiology 158, 1942-1952 (2012)
  31. Hirokazu Suzuki, Shunji Takahashi, Hiroyuki Osada, Ken-ichi Yoshida. Improvement of transformation efficiency by strategic circumvention of restriction barriers in Streptomyces griseus. J. Microbiol. Biotechnol. 21, 675-678 (2011)
  32. Naoki Kato, Hirokazu Suzuki, Hiroshi Takagi, Masakazu Uramoto, Shunji Takahashi, Hiroyuki Osada. Gene disruption and biochemical characterization of verruculogen synthase of Aspergillus fumigatus. ChemBioChem 12, 711-714 (2011)
  33. Naoki Kato*, Hirokazu Suzuki*, Hiroshi Takagi, Yukihiro Asami, Hideaki Kakeya, Masakazu Uramoto, Takeo Usui, Shunji Takahashi, Yoshikazu Sugimoto, Hiroyuki Osada. Identification of cytochrome P450s required for fumitremorgin biosynthesis in Aspergillus fumigatus. ChemBioChem 10, 920-928 (2009); *equal contribution
  34. Hirokazu Suzuki, Kazuya Marushima, Yasuo Ohnishi, Sueharu Horinouchi. A novel pair of terminal protein and telomere-associated protein for replication of the linear chromosome of Streptomyces griseus IFO 13350. Biosci. Biotechnol. Biochem. 72, 2973-2980 (2008)
  35. Yasuo Ohnishi, Jun Ishikawa, Hirofumi Hara, Hirokazu Suzuki, Miwa Ikenoya, Haruo Ikeda, Atsushi Yamashita, Masahira Hattori, Sueharu Horinouchi. The genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. J. Bacteriol 190, 4050-4060 (2008)
  36. Hirokazu Suzuki, Tokuzo Nishino, Toru Nakayama. Anthocyanin acyltransferase engineered for the synthesis of a novel polyacylated anthocyanin. Plant Biotechnol. 24, 495-501 (2007)
  37. Hirokazu Suzuki, Tokuzo Nishino, Toru Nakayama. cDNA cloning of a BAHD acyltransferase from soybean (Glycine max): isoflavone 7-O-glucoside-6’’-O-malonyltransferase. Phytochemistry 68, 2035-2042 (2007)
  38. Hirokazu Suzuki, Yasuo Ohnishi, Sueharu Horinouchi. GriC and GriD constitute a carboxylic acid reductase involved in grixazone biosynthesis in Streptomyces griseus. J. Antibiot. 60, 380-387 (2007)
  39. Hideaki Unno*, Fumiko Ichimaida*, Hirokazu Suzuki*, Seiji Takahashi, Yoshikazu Tanaka, Atsushi Saito, Tokuzo Nishino, Masami Kusunoki, Toru Nakayama. Structural and mutational studies of anthocyanin malonyltransferases establish the features of BAHD enzyme catalysis. J. Biol. Chem. 282, 15812-15822 (2007); *equal contribution
  40. Hirokazu Suzuki, Yasuo Ohnishi, Sueharu Horinouchi. Arylamine N-acetyltransferase responsible for acetylation of 2-aminophenols in Streptomyces griseus. J. Bacteriol. 189, 2155-2159 (2007)
  41. Hirokazu Suzuki, Yasuo Ohnishi, Yasuhide Furusho, Shohei Sakuda, Sueharu Horinouchi. Novel benzene ring biosynthesis from C3 and C4 primary metabolites by two enzymes. J. Biol. Chem. 281, 36944-36951 (2006); this paper has been selected as a JBC Papers of the week.
  42. Hirokazu Suzuki, Seiji Takahashi, Ryoko Watanabe, Yusuke Fukushima, Naoki Fujita, Akio Noguchi, Ryusuke Yokoyama, Kazuhiro Nishitani, Tokuzo Nishino, Toru Nakayama. An isoflavone conjugate-hydrolyzing β-glucosidase from the roots of soybean (Glycine max) seedlings: purification, gene cloning, phylogenetics, and cellular localization. J. Biol. Chem. 281, 30251-30259 (2006)
  43. Hirokazu Suzuki, Yasuhide Furusho, Tatsuichiro Higashi, Yasuo Ohnishi, Sueharu Horinouchi. A novel o-aminophenol oxidase responsible for formation of the phenoxazinone chromophore of grixazone. J. Biol. Chem. 281, 824-833 (2006)
  44. Shin’ya Sawada, Hirokazu Suzuki, Fumiko Ichimaida, Masa-atsu Yamaguchi, Takashi Iwashita, Yuko Fukui, Hisashi Hemmi, Tokuzo Nishino, Toru Nakayama. UDP-glucuronic acid: anthocyanin glucuronosyltransferase from red daisy (Bellis perennis) flowers. J. Biol. Chem. 280, 899-906 (2005)
  45. Hirokazu Suzuki, Shin’ya Sawada, Kazufumi Watanabe, Shiro Nagae, Masa-atsu Yamaguchi, Toru Nakayama, Tokuzo Nishino. Identification and characterization of a novel anthocyanin malonyltransferase from scarlet sage (Salvia splendens) flowers: an enzyme that is phylogenetically separated from other anthocyanin acyltransferases. Plant J. 38, 994-1003 (2004)
  46. Hirokazu Suzuki, Toru Nakayama, Shiro Nagae, Masa-atsu Yamaguchi, Takashi Iwashita, Yuko Fukui, Tokuzo Nishino. cDNA cloning and functional characterization of flavonol 3-O-glucoside-6’’-O-malonyltransferases from flowers of Verbena hybrida and Lamium purpureum. J. Mol. Cat. B: Enzymatic 28, 87-93 (2004)
  47. Hirokazu Suzuki, Toru Nakayama, Masa-atsu Yamaguchi, Tokuzo Nishino. cDNA cloning and characterization of two Dendranthema x morifolium anthocyanin malonyltransferases with different functional activities. Plant Sci. 166, 89-96 (2004)
  48. Hirokazu Suzuki, Shin’ya Sawada, Keiko Yonekura-Sakakibara, Toru Nakayama, Masa-atsu Yamaguchi, Tokuzo Nishino. Identification of a cDNA encoding malonyl-coenzyme A: anthocyanidin 3-O-gucoside 6”-O-malonyltransferase from cineraria (Senecio cruentus) flowers. Plant Biotechnol. 20, 229-234 (2003)
  49. Hirokazu Suzuki, Toru Nakayama, Tokuzo Nishino. Proposed mechanism and functional amino acid residues of malonyl-CoA: anthocyanin 5-O-glucoside-6'''-O-malonyltransferase from flowers of Salvia splendens, a member of the versatile plant acyltransferase family. Biochemistry 42, 1764-1771 (2003)
  50. Hirokazu Suzuki, Toru Nakayama, Keiko Yonekura-Sakakibara, Yuko Fukui, Noriko Nakamura, Masa-atsu Yamaguchi, Yoshikazu Tanaka, Takaaki Kusumi, Tokuzo Nishino. cDNA cloning, heterologous expressions, and functional characterization of malonyl-coenzyme A: anthocyanidin 3-O-glucoside-6"-O-malonyltransferase from dahlia flowers. Plant Physiol. 130, 2142-2151 (2002)
  51. Hirokazu Suzuki, Toru Nakayama, Keiko Yonekura-Sakakibara, Yuko Fukui, Noriko Nakamura, Masahiro Nakao, Yoshikazu Tanaka, Masa-atsu Yamaguchi, Takaaki Kusumi, Tokuzo Nishino. Malonyl-CoA: anthocyanin 5-O-glucoside-6’’’-O-malonyltransferase from scarlet sage (Salvia splendens) flowers. J. Biol. Chem. 276, 49013-49019 (2001)
総説・解説
  1. 鈴木 宏和. 好熱菌における耐熱化酵素の発生と選択ならびに進化工学への応用. 化学工業 73, pp.634-640 (2022)
  2. 鈴木 宏和. 中等度好熱菌を利用してタンパク質をつくる. バイオインダストリー 11, pp.15-21 (2021)
  3. Hirokazu Suzuki. Remarkable diversification of bacterial azoreductases: primary sequences, structures, substrates, physiological roles, and biotechnological applications. Appl. Microbiol. Biotechnol. 103, 3965-3978 (2019)
  4. Hirokazu Suzuki. Peculiarities and biotechnological potential of environmental adaptation by Geobacillus species. Appl. Microbiol. Biotechnol. 102, 10425-10437 (2018)
  5. 鈴木 宏和, 小林 淳平. 耐熱化酵素を好熱菌細胞内で創る. 化学と生物 52, pp.428-430 (2014)
  6. 鈴木 宏和. 高温バイオプロセスを志向した好熱菌の遺伝子工学. 極限環境生物学会誌 10, pp.69-76 (2011)
  7. Toru Nakayama, Hirokazu Suzuki, Tokuzo Nishino. Anthocyanin acyltransferases: specificities, mechanism, phylogenetics, and applications. J. Mol. Cat. B: Enzymatic 23, 117-132 (2003)
著書
  1. Keisuke Wada, Hirokazu Suzuki. Biotechnological platforms of the moderate thermophiles, Geobacillus species: notable properties and genetic tools. In: Physiological and Biotechnological Aspects of Extremophiles (edited by Richa Salwan and Vivek Sharma), Elsevier, Amsterdam, Netherlands, pp. 195-218 (2020)
  2. Hirokazu Suzuki. Enzymatic strategies for cleaving glycosidic linkages. In: Advances in Chemistry Research (edited by James C. Taylor), Nova Science Publishers, New York, USA, vol.40, pp.173-196 (2017)
  3. Tatsuki Mizuno, Takashi Ohshiro, Hirokazu Suzuki. Plasmid curing is a promising approach to improve thermophiles for biotechnological applications: perspectives in archaea. In: Archaea - New Biocatalysts, Novel Pharmaceuticals and Various Biotechnological Applications (edited by Haitham Sghaier), InTech, Rijeka, Croatia, pp.83-99 (2017)
  4. Jyumpei Kobayashi, Hirokazu Suzuki. Microbial responses and resistance to chloramphenicol. In: Advances in Medicine and Biology (edited by Leon V. Berhardt), Nova Science Publishers, New York, USA, vol.123, pp.53-91 (2017)
  5. Hirokazu Suzuki. Geobacillus kaustophilus HTA426: a model organism for moderate thermophiles. In: Advances in Medicine and Biology (edited by Leon V. Berhardt), Nova Science Publishers, New York, USA, vol.114, pp.75-108 (2017)
  6. Hirokazu Suzuki. Motif-based exploration of glycoside hydrolases family 1. In: Recent Advances in Ginseng and Glycosides Research (edited by Claude J. Hopkins), Nova Science Publishers, New York, USA, pp.119-136 (2013)
  7. Hirokazu Suzuki. Host-mimicking strategies in DNA methylation for improved bacterial transformation. In: Methylation - From DNA, RNA and Histones to Diseases and Treatment (edited by Anica Dricu), InTech, Rijeka, Croatia, pp.219-236 (2012)
  8. 鈴木 宏和, 大島 敏久. 高温バイオプロセスのための好熱菌宿主の開発. 極限環境生物の産業展開（今中 忠行 監修）, シーエムシー出版, pp.153-159 (2012)
紀要
  1. 鈴木 宏和. 高変異性好熱菌を利用した耐熱化変異酵素のハイスループット創出. IFO Res. Commun. 33, pp.198 (2019)
  2. 鈴木 宏和. 中等度好熱菌のスキマ研究を模索して. 鳥取大学工学部研究報告 49, pp.22-32 (2018)
  3. 鈴木 宏和. 耐熱化変異酵素を簡便かつ汎用的にスクリーニングする手法の開発. 公益財団法人長瀬化学技術財団研究報告集 28, pp.99-107 (2017)
  4. 石野 園子，鈴木 宏和，河原林 裕. 極限環境微生物遺伝子資源の応用. IFO Res. Commun. 30, pp.103-117 (2016)
  5. Jyumpei Kobayashi, Misaki Tanabiki, Megumi Furukawa, Hisashi Yagi, Takashi Ohshiro, Hirokazu Suzuki. Generation of chloramphenicol resistance plasmids functional at high temperatures by using an error-prone thermophile. Conference Proceedings of International Biotechnology, Chemical Engineering and Life Science Conference 2014 (IBCELC), pp.66-73 (2014)
  6. Hirokazu Suzuki, Jyumpei Kobayashi, Keisuke Wada, Megumi Furukawa, Katsumi Doi. Generation of thermostable enzyme genes using spontaneous mutations in thermophile Geobacillus kaustophilus HTA426. Industrial, Medical and Environmental Applications of Microorganisms: Current Status and Trends (edited by A. Mendez-Vilas), Wageningen Academic Publishers, Wageningen, Netherlands, pp.441-446 (2014)
受賞
  1. 平成29年度 鳥取大学科学研究業績表彰
  2. 平成28年度 日本農芸化学会中四国支部奨励賞
  3. 平成28年度 酵素応用シンポジウム研究奨励賞
  4. 平成28年度 長瀬研究振興賞
  5. 平成15年度 東北大学総長賞
  6. 平成15年度 東北大学工学研究科長賞
  7. 平成15年度 日本植物細胞分子生物学会 学生奨励賞
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E-mail ohshiro[at]tottori-u.ac.jp