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八木 寿梓（やぎ ひさし）
1977年9月9日 生まれ，大阪府出身，博士（再生医科学）
E-mail:yagi[at]tottori-u.ac.jp

準備中

経歴
2001年 3月  　鳥取大学 工学部 生物応用工学科 卒業
2003年 3月  　鳥取大学大学院 工学研究科 生物応用工学専攻 博士前期課程 修了
2006年 3月  　鳥取大学大学院 医学系研究科 機能再生医科学専攻 博士後期課程 修了
  　博士（再生医科学）
2006年 4月  　大阪大学 蛋白質研究所 特任研究員
2009年 4月  　日本学術振興会特別研究員（PD）（大阪大学）
2010年 1月  　大阪大学 蛋白質研究所 助教
2014年 3月  　鳥取大学工学部附属 GSCセンター テニュアトラック助教
2018年 4月  　鳥取大学大学院 工学研究科 准教授（現職）
所属学会
  1. 日本蛋白質科学会
  2. 日本生化学会
  3. 日本農芸化学会
原著論文
  1. Yoshihito Arai, Yunako Shingu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Occurrence of different fucoidanase genes in Flavobacterium sp. SW and enzyme characterization. J. Biosci. Bioeng. 134, 187-194 (2022)
  2. Takuto Shimizu, Emiko Nogami, Yuka Ito, Kazuo Morikawa, Masaki Nagane, Tadashi Yamashita, Tsuyoshi Ogawa, Fuyuki Kametani, Hisashi Yagi, Naomi Hachiya. Volatile anesthetic sevoflurane precursor 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) exerts an anti-prion activity in prion-infected culture cells. Neurochem. Res. 46, 2056-2065 (2021)
  3. Keiko Fujimori, Satoru Fujii, Lisa Lindiana, Satoshi Wakai, Hisashi Yagi, Yoshihiro Sambongi. Differences in biochemical properties of two 5'-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions. Biosci. Biotechnol. Biochem. 83, 1085-1093 (2019)
  4. Norihito Uemura, Hisashi Yagi, Maiko T. Uemura, Yusuke Hatanaka, Hodaka Yamakado, Ryosuke Takahashi. Inoculation of α-synuclein preformed fibrils into the mouse gastrointestinal tract induces Lewy body-like aggregates in the brainstem via the vagus nerve. Mol. Neurodegener. 13, 21 (2018)
  5. Tatsuhiko Nagao, Yoshihito Arai, Mika Yamaoka, Fumika Komatsu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Identification and characterization of the fucoidanase gene from Luteolibacter algae H18. J. Biosci. Bioeng. 126, 567-572 (2018)
  6. Kenta Fujii, Yurie Tominaga, Jyumpei Okunaka, Hisashi Yagi, Takashi Ohshiro, Hirokazu Suzuki. Microbial and genomic characterization of Geobacillus thermodenitrificans OS27, a marine thermophile that degrades diverse raw seaweeds. Appl. Microbiol. Biotechnol. 102, 4901-4913 (2018)
  7. Hisashi Yagi, Asako Fujise, Narumi Itabashi, Takashi Ohshiro. Characterization of a novel endo-type alginate lyase derived from Shewanella sp. YH1. J. Biochem. 163, 341-350 (2018)
  8. Hisashi Yagi, Natsuki Isobe, Narumi Itabashi, Asako Fujise, Takashi Ohshiro. Characterization of a long-lived alginate lyase derived from Shewanella species YH1. Mar. Drugs 16, 1-12 (2018)
  9. Tomoya Hino, Haruka Hamamoto, Hirokazu Suzuki, Hisashi Yagi, Takashi Ohshiro, Shingo Nagano. Crystal structures of TdsC, a dibenzothiophene monooxygenase from the thermophile Paenibacillus sp. A11-2, reveal potential for expanding its substrate selectivity. J. Biol. Chem. 292, 15804-15813 (2017)
  10. Tatsuhiko Nagao, Ayako Kumabe, Fumika Komatsu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Gene identification and characterization of fucoidan deacetylase for potential application to fucoidan degradation and diversification. J. Biosci. Bioeng. 124, 277-282 (2017)
  11. Hisashi Yagi, Asako Fujise, Narumi Itabashi, Takashi Ohshiro. Purification and characterization of a novel alginate lyase from the marine bacterium Cobetia sp. NAP1 isolated from brown algae. Biosci. Biotechnol. Biochem. 80, 2338-2346 (2016)
  12. Hisashi Yagi, Aiko Mizuno, Masatomo So, Miki Hirano, Masayuki Adachi, Yoko Akazawa-Ogawa, Yoshihisa Hagihara, Tatsuya Ikenoue, Young-Ho Lee, Yasushi Kawata, Yuji Goto. Ultrasonication-dependent formation and degradation of α-synuclein amyloid fibrils. Biochim. Biophys. Acta 1854, 209-217 (2015)
  13. Mayu Terakawa-Suzuki, Hisashi Yagi, Masayuki Adachi, Young-Ho Lee, Yuji Goto. Small liposomes accelerate the fibrillation of amyloid β (1-40). J. Biol. Chem. 290, 815-826 (2015)
  14. Hirotsugu Ogi, Masahiko Fukukshima, Hiroki Hamada, Kentaro Noi, Masahiko Hirao, Hisashi Yagi, Yuji Goto. Ultrafast propagation of β-amyloid fibrils in oligomeric cloud. Sci. Rep. 4, 6960 (2014)
  15. Ayaka Umemoto*, Hisashi Yagi*, Masatomo So*, Yuji Goto. High-throughput analysis of ultrasonication-forced amyloid fibrillation reveals the mechanism underlying the large fluctuation in the lag time. J. Biol. Chem. 289, 27290-27299 (2014); *equal contribution
  16. Cardine N. Nokwe, Martin Zacharias, Hisashi Yagi, Manuel Hora, Bernd Reif, Yuji Goto, Johannes Buchner. A residue-specific shift in stability and　amyloidogenicity of antibody variable domains. J. Biol. Chem. 289, 26829-26846 (2014)
  17. Hisashi Yagi*, Yuki Abe*, Naoto Takayanagi, Yuji Goto. Elongation of amyloid fibrils through lateral binding of monomers revealed by total internal reflection fluorescence microscopy. Biochim. Biophys. Acta 1844, 1881-1888 (2014); *equal contribution
  18. Jin Chen*, Hisashi Yagi*, Yuji Furutani, Takashi Nakamura, Asumi Inaguma, Hao Guo, Yan Kong, Yuji Goto. Self-assembly of the chaperonin GroEL nanocage induced at submicellar detergent. Sci. Rep. 4, 5614 (2014); *equal contribution
  19. Tatsuya Ikenoue, Young-Ho Lee, Jozsef Kardos, Miyu Saiki, Hisashi Yagi, Yasushi Kawata, Yuji Goto. Cold denaturation of α-synuclein amyloid fibrils. Angew. Chem. Int. Ed. Eng. 53, 7799-7804 (2014)
  20. Hiroya Muta, Young-Ho Lee, Jozsef Kardos, Yuxi Lin, Hisashi Yagi, Yuji Goto. Supersaturation-limited amyloid fibrillation of insulin revealed by ultrasonication. J. Biol. Chem. 289, 18228-18238 (2014)
  21. Tatsuya Ikenoue, Young-Ho Lee, Jozsef Kardos, Hisashi Yagi, Takahisa Ikegami, Hironobu Naiki, Yuji Goto. Heat of supersaturation-limited amyloid burst directly monitored by isothermal titration calorimetry. Proc. Natl. Acad. Sci. USA. 109, 6654-6659 (2014)
  22. Yuxi Lin, Young-Ho Lee, Yuichi Yoshimura, Hisashi Yagi, Yuji Goto. Solubility and supersaturation-dependent protein misfolding revealed by ultrasonication. Langmuir, 1845-1854 (2014)
  23. Hiroki Kitayama, Yuichi Yoshimura, Masatomo So, Kazumasa Sakurai, Hisashi Yagi, Yuji Goto. A common mechanism underlying amyloid fibrillation and protein crystallization revealed by the effects of ultrasonication. Biochim. Biophys. Acta 1834, 2640-2646 (2013)
  24. Hisashi Yagi, Kyohei Hasegawa, Yuichi Yoshimura, Yuji Goto. Acceleration of the depolymerization of amyloid β fibrils by ultrasonication. Biochim. Biophys. Acta 1834, 2480-2485 (2013)
  25. Patrizia P. Mangione, Gennaro Esposito, Annalisa Relini, Sara Raimondi, Riccardo Porcari, Sofia Giorgetti, Alessandra Corazza, Federico Fogolari, Amanda Penco, Yuji Goto, Young-Ho Lee, Hisashi Yagi, Ciro Cecconi, Mohsin M. Naqvi, Julian D. Gillmore, Philip N. Hawkins, Fabrizio Chiti, Ranieri Rolandi, Graham W. Taylor, Mark B. Pepys, Monica Stoppini, Vittorio Bellotti. Structure, folding dynamics and amyloidogenesis of D76N β2-microglobulin: roles of shear flow, hydrophobic surfaces, and α crystalline. J. Biol. Chem. 288, 30917-30930 (2013)
  26. Yusuke Kato*, Hisashi Yagi*, Yuichi Kaji, Tetsuro Oshika, Yuji Goto. Benzalkonium chloride accelerates the formation of the amyloid fibrils of corneal dystrophy-associated peptides. J. Biol. Chem. 288, 25109-25118 (2013); *equal contribution
  27. Huong Thi Vu, Toshinori Shimanouchi, Daisuke Ishikawa, Tadaharu Matsumoto, Hisashi Yagi, Yuji Goto, Hiroshi Umakoshi, Ryoichi Kuboi. Effect of liposome membranes on disaggregation of amyloid β fibrils by dopamine. Biochem. Eng. J. 71, 118-126 (2013)
  28. Kentaro Uesugi, Hirotsugu Ogi, Masahiko Fukushima, Masatomo So, Hisashi Yagi, Yuji Goto, Masahiko Hirao. Mechanism of ultrasonically induced fibrillation of amyloid β 1-40 peptides. Jpn. J. Appl. Phys. 52, 07HE10, 1-6 (2013)
  29. Hirotsugu Ogi, Masahiko Fukushima, Kentaro Uesugi, Hisashi Yagi, Yuji Goto, Masahiko Hirao. Acceleration of deposition of Aβ(1-40) peptide on ultrasonically formed Aβ(1-42) nucleus studied by wireless quartz-crystal-microbalance biosensor. Biosens. Bioelectron. 40, 200-205 (2013)
  30. Yasutaka Izawa, Hironobu Tateno, Hiroshi Kameda, Kazuya Hirakawa, Keiko Hato, Hisashi Yagi, Kunihiro Hongo, Tomohiro Mizobata, Yasushi Kawata. Role of C-terminal negative charges and tyrosine residues in fibril formation of α-synuclein. Brain Behav. 2, 595-605 (2012)
  31. Yuichi Yoshimura, Yuxi Lin, Hisashi Yagi, Young-Ho Lee, Hiroki Kitayama, Kazumasa Sakurai, Masatomo So, Hirotsugu Ogi, Hironobu Naiki, Yuji Goto. Distinguishing crystal-like amyloid fibrils and glass-like amorphous aggregates from their kinetics of formation. Proc. Natl. Acad. Sci. USA. 109, 14446-14451 (2012)
  32. Toshinori Shimanouchi, Naoya Shimauchi, Ryo Ohnishi, Nachi Kitaura, Hisashi Yagi, Yuji Goto, Hiroshi Umakoshi, Ryoichi Kuboi. Formation of spherulitic amyloid β aggregate by anionic liposomes. Biochem. Biophys. Res. Commun. 426, 165-171 (2012)
  33. Jin Chen*, Hisashi Yagi*, Pietro Sormanni, Michele Vendruscolo, Koki Makabe, Takashi Nakamura, Yuji Goto, Kunihiro Kuwajima. Fibrillogenic propensity of the GroEL apical domain: a Janus-faced minichaperone. FEBS Lett. 586, 1120-1127 (2012); *equal contribution
  34. Eri Chatani, Hisashi Yagi, Hironobu Naiki, Yuji Goto. Polymorphism of β2-microglobulin amyloid fibrils manifested by ultrasonication-enhanced fibril formation in trifluoroethanol. J. Biol. Chem. 287, 22827-22837 (2012)
  35. Masatomo So, Hisashi Yagi, Kazumasa Sakurai, Hirotsugu Ogi, Hironobu Naiki, Yuji Goto. Ultrasonication-dependent acceleration of amyloid fibril formation. J. Mol. Biol. 412, 568-577 (2011)
  36. Viktoria Babenko, Takunori Harada, Hisashi Yagi, Yuji Goto, Reiko Kuroda, Wojciech Dzwolak. Chiral superstructures of insulin amyloid fibrils. Chirality 23, 638-646 (2011)
  37. Kotaro Yanagi, Mizue Ashizaki, Hisashi Yagi, Kazumasa Sakurai, Young-Ho Lee, Yuji Goto. Hexafluoroisopropanol induces amyloid fibrils of islet amyloid polypeptide by enhancing both hydrophobic and electrostatic interactions. J. Biol. Chem. 286, 23959-23966 (2011)
  38. Hirotsugu Ogi, Yuji Fukunishi, Taiji Yanagida, Hisashi Yagi, Yuji Goto, Masahiko Fukushima, Kentaro Uesugi, Masahiko Hirao. Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor. Anal. Chem. 83, 4982-4988 (2011)
  39. Daisaku Ozawa, Yuichi Kaji, Hisashi Yagi, Kazumasa Sakurai, Toru Kawakami, Hironobu Naiki, Yuji Goto. Destruction of amyloid fibrils of Keratoepithelin peptides by laser irradiation coupled with amyloid-specific thioflavin T. J. Biol. Chem. 286, 10856-10863 (2011)
  40. Chai L. Teoh, Hisashi Yagi, Michael D. W. Griffin, Yuji Goto, Geoffrey J. Howlett. Visualization of polymorphism in apolipoprotein C-II amyloid fibrils. J. Biochem. 149, 67-74 (2011)
  41. Takahiro Yamaguchi, Hisashi Yagi, Yuji Goto, Katsumi Matsuzaki, Masaru Hoshino. A disulfide-linked amyloid-β peptide dimer forms a protofibril-like oligomer through a distinct pathway from amyloid fibril formation. Biochemistry 49, 7100-7107 (2010)
  42. Hisashi Yagi*, Hideki Takeuchi*, Shiho Ogawa, Naomi Ito, Isao Sakane, Kunihiro Hongo, Tomohiro Mizobata, Yuji Goto, Yasushi Kawata. Isolation of short peptide fragments from α-synuclein fibril core identifies a residue important for fibril nucleation: a possible implication for diagnostic applications. Biochim. Biophys. Acta 1804, 2077-2087 (2010); *equal contribution
  43. Jesper Ferkinghoff-Borg, Jesper Fonslet, Christian Beyschau Andersen, Sandeep Krishna, Simone Pigolotti, Hisashi Yagi, Yuji Goto, Daniel Otzen, Mogens H. Jensen. Stop-and-go kinetics in amyloid fibrillation. Phys. Rev. E 82, 010901(R) (2010)
  44. Kaori Yamamoto, Hisashi Yagi, Young-Ho Lee, Jozsef Kardos, Yoshihisa Hagihara, Hironobu Naiki, Yuji Goto. The amyloid fibrils of the constant domain of immunoglobulin light chain. FEBS Lett. 584, 3348-3353 (2010)
  45. Hisashi Yagi, Daisaku Ozawa, Kazumasa Sakurai, Toru Kawakami, Hiroki Kuyama, Osamu Nishimura, Toshinori Shimanouchi, Ryoichi Kuboi, Hironobu Naiki, Yuji Goto. Laser-induced propagation and destruction of amyloid fibrils. J. Biol. Chem. 285, 19660-19667 (2010)
  46. Toshinori Shimanouchi, Naoya Shimauchi, Keiichi Nishiyama, Huong Thi Vu, Hisashi Yagi, Yuji Goto, Hiroshi Umakoshi, Ryoichi Kuboi. Characterization of amyloid-β fibrils with an aqueous two-phase system: implications of fibril formation. Solvent Extr. Res. Dev., Jpn. 17, 121-128 (2010)
  47. Vu Thi Huong, Toshinori Shimanouchi, Naoya Shimauchi, Hisashi Yagi, Hiroshi Umakoshi, Yuji Goto, Ryoichi Kuboi. Catechol derivatives inhibit the fibril formation of amyloid-β peptides. J. Biosci. Bioeng. 109, 629-634 (2010)
  48. Eri Chatani, Young-Ho Lee, Hisashi Yagi, Yuichi Yoshimura, Hironobu Naiki, Yuji Goto. Ultrasonication-dependent production and breakdown lead to minimum-sized amyloid fibrils. Proc. Natl. Acad. Sci. USA. 106, 11119-11124 (2009)
  49. Kenji Sasahara, Hisashi Yagi, Hironobu Naiki, Yuji Goto. Thermal response with exothermic effects of 2-microglobulin amyloid fibrils and fibrillation. J. Mol. Biol. 389, 584-594 (2009)
  50. Christian Beyschau Andersen, Hisashi Yagi, Mauro Manno, Vincenzo Martorana, Tadato Ban, Gunna Christiansen, Daniel Erik Otzen, Yuji Goto, Christian Rischel. Branching in amyloid fibril growth. Biophys. J. 96, 1529-1536 (2009)
  51. Daisaku Ozawa, Hisashi Yagi, Tadato Ban, Atsushi Kameda, Toru Kawakami, Hironobu Naiki, Yuji Goto. Destruction of amyloid fibrils of a 2-microglobulin fragment by laser beam irradiation. J. Biol. Chem. 284, 1009-1017 (2009)
  52. Hisashi Yagi, Ai Sato, Akihiro Yoshida, Yoshiki Hattori, Masahiro Hara, Jun Shimamura, Isao Sakane, Kunihiro Hongo, Tomohiro Mizobata, Yasushi Kawata. Fibril formation of hsp10 homologue proteins and determination of fibril core regions: differences in fibril core regions dependent on subtle differences in amino acid sequence. J. Mol. Biol. 377, 1593-1606 (2008)
  53. Kenji Sasahara, Hisashi Yagi, Miyo Sakai, Hironobu Naiki, Yuji Goto. Amyloid nucleation triggered by agitation of 2-microglobulin under acidic and neutral pH conditions. Biochemistry 47, 2650-2660 (2008)
  54. Kaori Yamamoto, Hisashi Yagi, Daisaku Ozawa, Kenji Sasahara, Hironobu Naiki, Yuji Goto. Thiol compounds inhibit the formation of amyloid fibrils by 2-microglobulin at neutral pH. J. Mol. Biol. 376, 258-268 (2008)
  55. Hisashi Yagi, Tadato Ban, Kenichi Morigaki, Hironobu Naiki, Yuji Goto. Visualization and classification of amyloid supramolecular assemblies. Biochemistry 46, 15009-15017 (2007)
  56. Kenji Sasahara, Hisashi Yagi, Hironobu Naiki, Yuji Goto. Heat-induced conversion of 2-microglobulin and hen egg-white lysozyme into amyloid fibrils. J. Mol. Biol. 372, 981-991 (2007)
  57. Rumi Adachi, Kei-ichi Yamaguchi, Hisashi Yagi, Kazumasa Sakurai, Hironobu Naiki, Yuji Goto. Flow-induced alignment of amyloid protofilaments revealed by linear dichroism. J. Biol. Chem. 282, 8978-8983 (2007)
  58. Kenji Sasahara, Hisashi Yagi, Hironobu Naiki, Yuji Goto. Heat-triggered conversion of protofibrils into mature amyloid fibrils of 2-microglobulin. Biochemistry 46, 3286-3293 (2007)
  59. Tadato Ban, Kenichi Morigaki, Hisashi Yagi, Takashi Kawasaki, Atsuko Kobayashi, Syunsuke Yuba, Hironobu Naiki, Yuji Goto. Real-time and single fibril observation of the formation of amyloid spherulitic structures. J. Biol. Chem. 281, 33677-33683 (2006)
  60. Hisashi Yagi, Eiko Kusaka, Kunihiro Hongo, Tomohiro Mizobata, Yasushi Kawata. Amyloid fibril formation of α-synuclein is accelerated by preformed amyloid seeds of other proteins: implications for the mechanism of transmissible conformational diseases. J. Biol. Chem. 280, 38609-38616 (2005)
  61. Takashi Higurashi*, Hisashi Yagi*, Tomohiro Mizobata, Yasushi Kawata. Amyloid like fibril formation of co-chaperonin GroES: nucleation and extension prefer different degrees of molecular compactness. J. Mol. Biol. 351, 1057-1069 (2005); *equal contribution
総説・解説
  1. 宗 正智，八木 寿梓，後藤祐児．超音波による蛋白質のアミロイド線維形成・結晶化の促進． 生物物理 54, 297-302 (2014)
  2. 八木 寿梓，後藤 祐児. α-Synuclein（PARK1/4）の機能・構造・線維形成． 医学のあゆみ「遺伝子・再生医療研究から学ぶパーキンソン病」 247，93-998 (2013)
  3. 宗正 智，吉村 優一, 八木 寿梓，後藤 祐児. 超音波によるタンパク質のアミロイド線維形成反応の促進． ケミカルエンジニヤリング 58，55-61 (2013)
  4. Yuichi Yoshimura, Masatomo So, Hisashi Yagi, Yuji Goto. Ultrasonication: An efficient agitation for accelerating the supersaturation-limited amyloid fibrillation of proteins. Jpn. J. Appl. Phys., 52, 07HA01, 1-8 (2003)
  5. 八木 寿梓，後藤 祐児．アミロイドーシス発症の分子機構解明． Medical Bio 49(7), 30-35 (2010)
  6. Hisashi Yagi, Tadato Ban, Yuji Goto. Real-time observation of amyloid fibril growth by total internal reflection fluorescence microscopy. Water and Biomolecules. Physical Chemistry of Life Phenomena, 289-299 (2009)
  7. Yuji Goto, Hisashi Yagi, Keiichi Yamaguchi, Eri Chatani, Tadato Ban. Structure, formation and propagation of amyloid fibrils. Curr. Pharm. Des. 14, 3205-3218 (2008)
  8. 伴 匡人，八木 寿梓，後藤 祐児．アミロイド線維の全反射蛍光顕微鏡による観察法． 蛋白質科学会アーカイブ 1, e042 (2008)
  9. 八木 寿梓，櫻井 一正，後藤 祐児．アミロイド構造生物学の台頭． 蛋白質・核酸・酵素 52(12), 1445-1453 (2007)
受賞
  1. 2010年 Annual Report of Osaka University Academic Achievement 2010（大阪大学論文100選）
  2. 2008年 第1回「Merck Award for Young Biochemistry Researcher」最優秀賞
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E-mail ohshiro[at]tottori-u.ac.jp